Coding
PsPT1

Part:BBa_K4179001:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-09-18)


Pastinaca sativa prenyl transferase 1 (PsPT1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 127
    Illegal EcoRI site found at 562
    Illegal PstI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 127
    Illegal EcoRI site found at 562
    Illegal NheI site found at 1210
    Illegal PstI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 127
    Illegal EcoRI site found at 562
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 127
    Illegal EcoRI site found at 562
    Illegal PstI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 127
    Illegal EcoRI site found at 562
    Illegal PstI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for E. coli.

Source

Pastinaca Sativa (wild parsnip) [1].

References

1. Munakata R, Olry A, Karamat F et al. Molecular evolution of parsnip (Pastinaca sativa) membrane-bound prenyltransferases for linear and/or angular furanocoumarin biosynthesis. New Phytologist 2016; 211: 332–344.